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IHC <t>for</t> <t>TRK,</t> <t>TRKA,</t> TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.
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IHC <t>for</t> <t>TRK,</t> <t>TRKA,</t> TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.
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IHC <t>for</t> <t>TRK,</t> <t>TRKA,</t> TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.
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IHC <t>for</t> <t>TRK,</t> <t>TRKA,</t> TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.
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IHC for TRK, TRKA, TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of NTRK Fusions Detection Method in Esophageal Squamous Cell Carcinoma and Gastric Adenocarcinoma

doi: 10.3390/ijms27010336

Figure Lengend Snippet: IHC for TRK, TRKA, TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.

Article Snippet: After rinsing in PBS, the sections were incubated with ananti-pan-TRK antibody (# EPR17341 ; 1:50 dilution; Abcam, Cambridge, UK), anti-TRKA antibody (#2510; 12G8; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-TRKB antibody (#4607; 80G2; 1:2000 dilution; Cell signaling Technology), or anti-TRKC antibody (#3376; C44H5; 1:1000 dilution; Cell Signaling Technology) at 4 °C overnight.

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Negative Staining